Production of Doubled Haploids from Rice Hybrid KRH-2 through Anther Culture and their Evaluation for Agro-Morphological Traits


Swapnil Ravindra Kulkarni1 , Ulaganathan K2 , Sundaram RM1 , Hari Prasad AS1 , R. Abdul Fiyaz1 and Balachandran SM1*

1 ICAR-Indian Institute of Rice Research, Hyderabad, India.

2 Centre for Plant Molecular Biology, Osmania University, Hyderabad.

*Corresponding author (Email ID: ) 

Received: 10th April 2020; Accepted: 30th May 2020


The present study was aimed to optimize conditions of anther culture and production of doubled haploids of a popular rice hybrid, KRH-2. Immature paniclescollected from field grown donor plants were pre-treated at 8°C for eight days and plated on four different basic media for callus induction with different hormonal concentrations and supplements. N6 media with maltose as the carbon source, supplemented with 2 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg L-1 of kinetin and 10 mg L-1 of silver nitrate (designated as N6-4 medium)
was found to give the highest callus induction (22.4%). The compact calli, which were transferred to MS regeneration medium supplemented with 2.5 mg L-1 of benzyl amino purine (BAP), 0.5 mg L-1 of kinetin and 1.5 mg L-1 of naphthalene acetic acid (NAA) recorded a good regeneration (25.4%). A total of 125 stable, doubled haploid lines (DHLs) were produced through spontaneousdoubling of KRH-2 microspores. The lines showed significant variability with respect to key agro-morphological traits

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