Received : 13^th July, 2023; Accepted : 20^th August, 2023

Abstract

Phytic acid present in rice grain is an anti-nutrient that hinders the absorption of iron (Fe) and zinc (Zn) ions in the human digestive system. Estimation of phytic acid is a part of the biofortification studies targeted to enhance micronutrient content in the rice grain. Among the various methods of phytic acid estimation, colorimetric method is highly amenable to most of the laboratories due to its simple requirements. Wade reagent has been widely used in phytic acid estimation in various crops. However, details like the range of phytic acid concentrations required for standard graph preparation and maximum limits of detection were not clearly mentioned and the volume of Wade reagent differed. Hence, the objective of this study is to identify the range of phytic acid concentrations that is suitable to develop phytic acid standard graph followed by the determination of the maximum limit of Wade reagent volume. The results of this study will be useful in the future studies to understand the inherent details like the volume of Wade reagent, volume of biological extract, optimum volume as well as concentration optimum for standard graph preparation and the factor for calculating phytate-P from phytic acid.